TB-500 (5 mg)

At a Glance

TB-500 is a synthetic 7-amino acid fragment (Ac-LKKTETQ) of thymosin beta-4 that promotes systemic tissue repair by binding G-actin, driving cell migration and angiogenesis, and reducing inflammation across multiple tissue types.[1] A gradual dosing approach is used to build tissue levels before settling at a standard maintenance dose.

• Reconstitute: Add 3.0 mL bacteriostatic water → 1.67 mg/mL concentration.
• Standard dose: 750 mcg once daily subcutaneous injection.
• Easy measuring: At 1.67 mg/mL on a U-100 syringe, 1 unit = 0.01 mL = 16.7 mcg. A 750 mcg dose = 45 units / 0.45 mL.
• Storage: Lyophilised: freeze at −20 °C; reconstituted: refrigerate at 2–8 °C; use within 4 weeks.

Overview

• Goal: Systemic tissue repair via G-actin binding, cell migration facilitation, angiogenesis, anti-inflammatory, and anti-fibrotic signalling.[1]
• Schedule: Once daily subcutaneous injection.
• Dose: 500–1,000 mcg per injection (30–60 units / 0.30–0.60 mL); standard 750 mcg.
• Reconstitution: 3.0 mL BAC water per 5 mg vial → 1.67 mg/mL.
• Storage: Lyophilised at −20 °C; reconstituted at 2–8 °C; use within 4 weeks.

What You’ll Need

Plan based on a 30-day course at 750 mcg once daily (30 injections, 22.5 mg total).

• TB-500 Vials (5 mg each): 22.5 mg ÷ 5 mg per vial → 5 vials.
• Insulin Syringes (U-100, 1 mL): 30 injections → 30 syringes.
• Bacteriostatic Water (10 mL bottles): 3.0 mL per vial → 2 × 10 mL bottles.
• Alcohol Swabs: 2 per injection → 60 swabs for the 30-day course.

How to Reconstitute

1. Allow frozen vial to reach room temperature (10–15 minutes).
2. Draw 3.0 mL bacteriostatic water with a sterile syringe.
3. Inject slowly down the inner vial wall; do not inject directly onto the lyophilised cake.
4. Gently swirl until fully dissolved — do not shake. Solution should be clear and colourless.
5. Label with reconstitution date; refrigerate at 2–8 °C, protected from light. Use within 4 weeks.

Dosing Schedule

Reconstitute each 5 mg vial with 3.0 mL bacteriostatic water for a concentration of 1.67 mg/mL (1 unit on a U-100 syringe = 16.7 mcg). The schedule above titrates from 500 mcg/day up to 1,000 mcg/day over 12 weeks, allowing your body to build tissue levels gradually. TB-500 distributes systemically, so injection site proximity to the injury is less critical than with BPC-157.

Protocol Details

• Starting: 500 mcg (30 units / 0.30 mL) subcutaneous, once daily.[1]
• Standard: 750 mcg (45 units / 0.45 mL) subcutaneous, once daily.
• High: 1,000 mcg (60 units / 0.60 mL) subcutaneous, once daily.
• Injection site: Abdomen, thigh, or upper arm; TB-500 distributes systemically from any site.
• Duration: Assess healing progress at week 4; adjust dose based on response.

Storage

• Lyophilised: Store at −20 °C (−4 °F); protect from moisture and light.
• Reconstituted: Refrigerate at 2–8 °C. Do not freeze. Use within 4 weeks.
• Appearance: Clear, colourless solution. Discard if cloudy or particulate.

How TB-500 Works

TB-500 corresponds to amino acids 17–23 of thymosin beta-4 (Tβ4), a 43-amino acid protein constitutively expressed in platelets, macrophages, and most nucleated cells. Under injury, Tβ4 is released from activated platelets to initiate repair.[1]

TB-500 sequesters G-actin (monomeric actin), controlling the pool available for filament polymerisation and enabling rapid migration of fibroblasts, keratinocytes, and endothelial cells into wound sites. It recruits endothelial progenitor cells and upregulates VEGF signalling to drive angiogenesis, restoring blood supply to ischaemic tissue. It also down-regulates NF-κB activity and pro-inflammatory cytokines (IL-6, TNF-α) and modulates myofibroblast activity to reduce scar tissue formation — resulting in more organised, functional repair tissue.[1]

Good to Know

• TB-500 distributes systemically — injection proximity to injury site is not required, though abdomen or thigh are most convenient.
• WADA-prohibited (Category S2: Non-Approved Peptide Hormones) — athletes subject to anti-doping rules must not use TB-500.
• Most researchers combine TB-500 with BPC-157 (Wolverine Stack) for complementary local + systemic tissue repair. See the Wolverine Stack Protocol.
• Track pain scores, range of motion, and swelling at weeks 2, 4, and 8 to assess response.
• Multi-tissue repair: Preclinical evidence for healing in skeletal muscle, tendons, ligaments, cardiac muscle, cornea, skin, and neural tissue.[1]
• Angiogenesis: Tβ4 promotes endothelial progenitor cell recruitment and VEGF upregulation in ischaemia models.[2]
• Human wound data: Phase II trial of topical Tβ4 gel improved closure of chronic venous stasis ulcers vs. standard care.[3]
• Anti-fibrotic: Reduced scar tissue formation compared to untreated controls in multiple preclinical studies.
• WADA status: Prohibited in- and out-of-competition for athletes under anti-doping jurisdiction.
• For background on TB-500's mechanism, evidence, and safety profile, see What Is TB-500?.

Tips for Best Results

• Maintain adequate dietary protein (1.2–1.6 g/kg) to supply amino acids for tissue synthesis.
• Gentle range-of-motion exercises during the initial dosing phase support repair without re-injury risk.
• Adequate sleep (7–9 hours) maximises growth hormone release and tissue anabolism during recovery.
• Avoid NSAIDs during the active recovery phase — they can blunt prostaglandin-mediated repair signalling that TB-500 potentiates.

Injection Tips

• Clean the vial stopper and injection site with separate alcohol swabs; allow both to air-dry fully before proceeding.
• Using a 29–31 gauge insulin syringe (5/16″ to 1/2″ needle), draw the calculated dose precisely.
• Pinch a fold of skin and insert the needle at 45° into subcutaneous fat (90° is acceptable with a short needle into a well-pinched fold).
• Inject slowly over 2–3 seconds; do not aspirate. Withdraw the needle, apply gentle pressure, and do not rub the site.
• Rotate injection sites (abdomen, thighs, upper arms) and dispose of each syringe in a sharps container immediately after use.