TB-500 (10 mg)

At a Glance

TB-500 is a synthetic 7-amino acid peptide (Ac-LKKTETQ) corresponding to the active region of thymosin beta-4, a naturally occurring regenerative protein. It promotes tissue repair by binding G-actin monomers to enhance cellular motility, stimulating angiogenesis, and reducing inflammation.[1] The 10 mg vial provides approximately 10 days of peptide at the standard dose.

• Reconstitute: Add 3.0 mL bacteriostatic water → 3.33 mg/mL concentration.
• Standard dose: 500–1,000 mcg once daily subcutaneous injection.
• Easy measuring: At 3.33 mg/mL on a U-100 syringe, 1 unit = 0.01 mL = 33.3 mcg. A 500 mcg dose = 15 units / 0.15 mL.
• Storage: Lyophilised: freeze at −20 °C; reconstituted: refrigerate at 2–8 °C; use within 4 weeks.

Overview

• Goal: Promote tissue regeneration via enhanced cellular motility (G-actin binding), angiogenesis, and anti-inflammatory signalling.[1]
• Schedule: Once daily subcutaneous injection.
• Dose range: 500–1,000 mcg per injection (15–30 units / 0.15–0.30 mL).
• Reconstitution: 3.0 mL BAC water per 10 mg vial → 3.33 mg/mL.
• Injection route: Subcutaneous (preferred) or intramuscular.

What You’ll Need

Plan based on a 30-day course at 1,000 mcg once daily (30 injections, 30 mg total).

• TB-500 Vials (10 mg each): 30 mg needed ÷ 10 mg per vial → 3 vials.
• Insulin Syringes (U-100, 1 mL): 30 injections → 30 syringes.
• Bacteriostatic Water (10 mL bottles): 3.0 mL per vial → 1 × 10 mL bottle.
• Alcohol Swabs: 2 per injection → 60 swabs for the 30-day course.

How to Reconstitute

1. Allow frozen lyophilised vial to reach room temperature (10–15 minutes).
2. Draw 3.0 mL bacteriostatic water with a sterile syringe.
3. Inject slowly down the inner vial wall at a 45° angle to avoid foaming.
4. Gently swirl or roll until fully dissolved — never shake. Solution should be clear.
5. Label with reconstitution date; refrigerate at 2–8 °C, protected from light. Use within 4 weeks.

Dosing Schedule

Reconstitute with 3.0 mL bacteriostatic water for a concentration of 3.33 mg/mL, where 1 unit = 33.3 mcg on a U-100 syringe. Start low and titrate up every two weeks as you assess tolerance. TB-500 distributes systemically regardless of injection site, so you don’t need to inject near the injury — though some practitioners prefer proximal injection for acute issues. The 10 mg vial lasts roughly 10 days at the full dose (1,000 mcg/day) or 20 days at the starting dose (500 mcg/day).[1]

Protocol Details

• Starting: 500 mcg (15 units / 0.15 mL) subcutaneous, once daily.[1]
• Standard: 1,000 mcg (30 units / 0.30 mL) subcutaneous, once daily.
• Injection site: Abdomen, thigh, or upper arm; TB-500 distributes systemically from any site.
• Duration: Assess healing progress at week 4; adjust dose based on response.

Storage

• Lyophilised: Store at −20 °C; stable for up to 3 years frozen, 2 years refrigerated.
• Reconstituted: Refrigerate at 2–8 °C. Do not freeze. Use within 4 weeks.
• Appearance: Clear solution. Discard if cloudy, coloured, or particulate.

How TB-500 Works

TB-500 is a synthetic heptapeptide corresponding to amino acids 17–23 of thymosin beta-4, a 43-amino acid regenerative protein found in virtually all human cells. Its core mechanism involves binding to G-actin monomers, preventing their polymerisation into F-actin filaments. This keeps cells in a more motile state, enabling faster migration into damaged tissue.[1]

TB-500 also promotes angiogenesis (new blood vessel formation), activates satellite cell differentiation in skeletal muscle, and downregulates pro-inflammatory cytokines. Unlike BPC-157 which acts primarily at the local injection site, TB-500 distributes systemically via albumin binding, providing whole-body regenerative support. This complementary mechanism is why the two peptides are frequently combined in the Wolverine Stack.[2]

Good to Know

• TB-500 distributes systemically regardless of injection site — it does not need to be injected near the injury, unlike BPC-157.
• WADA-prohibited substance — competitive athletes subject to anti-doping rules must not use TB-500.
• TB-500 is often combined with BPC-157 for complementary systemic + local repair. See the Wolverine Stack Protocol.
• Initial effects (reduced inflammation) are typically noticed within 1–2 weeks; full regenerative benefits develop over 4–8 weeks.
• Temporary water retention and mild fatigue are common during the loading phase and typically resolve.
• Tissue regeneration: Promotes healing of tendons, ligaments, muscles, and chronic wounds through enhanced cellular motility and angiogenesis.[1]
• Anti-inflammatory: Reduces pro-inflammatory cytokines; may improve recovery from overtraining.
• Neuroprotective: Preclinical evidence of spinal cord and nerve tissue support.[2]
• Side effects: Fatigue, dizziness, headache, injection site irritation, temporary water retention — generally mild.
• Theoretical concern: Enhanced angiogenesis could be problematic in malignant conditions — avoid if history of cancer.
• For background on TB-500's mechanism, evidence, and safety profile, see What Is TB-500?.

Tips for Best Results

• Maintain adequate dietary protein (1.2–2.0 g/kg) to support tissue synthesis driven by TB-500.
• Progressively increase activity as healing permits — controlled loading stimulates aligned collagen deposition.
• Adequate sleep (7–9 hours) supports the anabolic repair environment.
• Stay well hydrated to help manage temporary water retention during loading phase.

Injection Tips

• Clean the vial stopper and injection site with separate alcohol swabs; allow both to air-dry fully before proceeding.
• Using a 29–31 gauge insulin syringe (5/16″ to 1/2″ needle), draw the calculated dose precisely.
• Pinch a fold of skin and insert the needle at 45° into subcutaneous fat (90° is acceptable with a short needle into a well-pinched fold).
• Inject slowly over 2–3 seconds; do not aspirate. Withdraw the needle, apply gentle pressure, and do not rub the site.
• Rotate injection sites (abdomen, thighs, upper arms) and dispose of each syringe in a sharps container immediately after use.